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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
05/04/2022 |
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Actualizado : |
01/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
SCHVARTZMAN, C.; FRESIA, P.; MURCHIO, S.; MUJICA, V.; DALLA RIZZA, M. |
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Afiliación : |
CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
RNAi in Piezodorus guildinii (Hemiptera: Pentatomidae): transcriptome assembly for the development of pest control strategies. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839 |
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Páginas : |
13 p. |
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DOI : |
10.3389/fpls.2022.804839 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022.
Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy
Al abrir el registro se visualiza el enlace al material suplementario. |
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Contenido : |
Red-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. |
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Palabras claves : |
DSRNA,; PEST CONTROL; RNA-SEQ; RNAI; STINK BUG; VATPASE A,. |
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Asunto categoría : |
H10 Plagas de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16649/1/Frontiers-Plant-Science-2022-Schvartzman.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/16650/1/Frontiers-Plant-Science-2022-Schvartzman-1.PDF
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Marc : |
LEADER 02536naa a2200277 a 4500
001 1062960
005 2022-09-01
008 2022 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2022.804839$2DOI
100 1 $aSCHVARTZMAN, C.
245 $aRNAi in Piezodorus guildinii (Hemiptera$bPentatomidae): transcriptome assembly for the development of pest control strategies.$h[electronic resource]
260 $c2022
300 $a13 p.
500 $aArticle history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022. Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy Al abrir el registro se visualiza el enlace al material suplementario.
520 $aRed-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies.
653 $aDSRNA,
653 $aPEST CONTROL
653 $aRNA-SEQ
653 $aRNAI
653 $aSTINK BUG
653 $aVATPASE A,
700 1 $aFRESIA, P.
700 1 $aMURCHIO, S.
700 1 $aMUJICA, V.
700 1 $aDALLA RIZZA, M.
773 $tFrontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839
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Registro original : |
INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
26/11/2019 |
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Actualizado : |
27/11/2019 |
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Tipo de producción científica : |
Presentaciones Orales |
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Autor : |
REYNO, R.; DALLA RIZZA, M.; CASTILLO, A.; DO CANTO, J.; CONDON, F.; MENESES, L.; LATTANZI, F.; MONZA, J. |
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Afiliación : |
RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JAVIER DO CANTO FAGUNDEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO CONDON PRIANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUCÍA VALERIA MENESES MEDINA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO A. LATTANZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE MONZA, Universidad de la República (UdelaR)/ Facultad de Agronomía. |
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Título : |
Estrategias de mejoramiento de forrajeras en Uruguay: enfrentando nuevos desafíos. [Presentación oral]. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
In: Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; Simposio RedBio Argentina, 11., Montevideo 12-15 noviembre, 2019. |
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Idioma : |
Español |
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Notas : |
Serie Técnica 253: Libro de Resúmenes. |
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Contenido : |
Estrategias complementarias. Diversidad genética en gramíneas nativas: Paspalum notatum. Diversidad genética en gramíneas nativas:
Bromus auleticus. Hibridación interespecífica: género Lotus. Rizobios de Trifolium mejor adaptados. Endófitos en Festuca. Endófitos en Bromus auleticus. Comentarios finales. |
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Palabras claves : |
FORRAJERAS. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13845/1/Presentacion-oral-redbio-2019.pdf
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Marc : |
LEADER 01063nam a2200217 a 4500
001 1060461
005 2019-11-27
008 2019 bl uuuu u00u1 u #d
100 1 $aREYNO, R.
245 $aEstrategias de mejoramiento de forrajeras en Uruguay$benfrentando nuevos desafíos. [Presentación oral].$h[electronic resource]
260 $aIn: Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; Simposio RedBio Argentina, 11., Montevideo 12-15 noviembre$c2019
500 $aSerie Técnica 253: Libro de Resúmenes.
520 $aEstrategias complementarias. Diversidad genética en gramíneas nativas: Paspalum notatum. Diversidad genética en gramíneas nativas: Bromus auleticus. Hibridación interespecífica: género Lotus. Rizobios de Trifolium mejor adaptados. Endófitos en Festuca. Endófitos en Bromus auleticus. Comentarios finales.
653 $aFORRAJERAS
700 1 $aDALLA RIZZA, M.
700 1 $aCASTILLO, A.
700 1 $aDO CANTO, J.
700 1 $aCONDON, F.
700 1 $aMENESES, L.
700 1 $aLATTANZI, F.
700 1 $aMONZA, J.
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INIA Tacuarembó (TBO) |
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